Production of transgenic carnation with antisense ACS (1-aminocyclopropane44-carboxy late synthase) gene

A tétel rövid nézete
dc.contributor.authorKiss, E.
dc.contributor.authorVeres, A.
dc.contributor.authorGalli, Zs.
dc.contributor.authorNagy, N.
dc.contributor.authorTóth, E.
dc.contributor.authorVarga, Á.
dc.contributor.authorHrazdina, G.
dc.contributor.authorHeszky, L.
dc.date.accessioned2021-06-28T09:57:47Z
dc.date.available2021-06-28T09:57:47Z
dc.date.issued2000-08-23
dc.description.abstractDianthus chinensis and Dianthus caryophyllus varieties were tested for shoot regeneration from leaf and petal explants and transformed with Agrobacterium tuniefaciens strains (EHA 105 and LBA 4404) harbouring an apple derived ACS cDNA in antisense orientation in order to reduce ethylene production and influence the ethylene dependant traits in carnation. After transformation regenerating shoots were selected on MS medium containing 50-75-100-125-150 mg/1 kanamycin and supplemented with 1 mg/1 BA, 0.2 mg/1 NAA. Transgene integration was proved by PCR analysis with npt II spcific primers followed by Southern hybridisation of DNA isolated from green shoots on medium containing 150 mg/1 kanamycin. Several putative transformants were subjected to RT-PCR in order to examine the npt 11 expression at mRNA level. Both the transformant and the non-transformant plants were potted into glasshouse to observe the effect of changed ethylene production on flowering time, petal senescence and vase life.  en
dc.formatapplication/pdf
dc.identifier.citationInternational Journal of Horticultural Science, Vol. 6 No. 4 (2000) , 104-107.
dc.identifier.doihttps://doi.org/10.31421/IJHS/6/4/235
dc.identifier.eissn2676-931X
dc.identifier.issn1585-0404
dc.identifier.issue4
dc.identifier.jatitleInt. j. hortic. sci.
dc.identifier.jtitleInternational Journal of Horticultural Science
dc.identifier.urihttps://hdl.handle.net/2437/314071en
dc.identifier.volume6
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/235
dc.rights.accessOpen Access
dc.rights.ownerInternational Journal of Horticultural Science
dc.subjectACSen
dc.subjectAgrobacterium tumefaciensen
dc.subjectDianthus caryophyllusen
dc.subjectDianthus chinensisen
dc.subjectethylene biosynthesisen
dc.subjectnpt IIen
dc.titleProduction of transgenic carnation with antisense ACS (1-aminocyclopropane44-carboxy late synthase) geneen
dc.typefolyóiratcikkhu
dc.typearticleen
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