Nicotinic acid suppresses sebaceous lipogenesis of human sebocytes via activating hydroxycarboxylic acid receptor 2 (HCA )

A tétel rövid nézete
dc.contributor.authorMarkovics, Arnold
dc.contributor.authorTóth, Kinga Fanni
dc.contributor.authorSós, Katalin Eszter
dc.contributor.authorMagi, József
dc.contributor.authorGyöngyösi, Adrienn
dc.contributor.authorBenyó, Zoltán
dc.contributor.authorZouboulis, Christos C.
dc.contributor.authorBíró, Tamás
dc.contributor.authorOláh, Attila
dc.contributor.authorOláh Attila (1984-) (élettanász)
dc.contributor.authorBiró Tamás (1968-) (élettanász)
dc.contributor.authorTóth Kinga Fanni (1992-) (Molekuláris biológus)
dc.contributor.authorMarkovics Arnold (1988-) (molekuláris biológus)
dc.contributor.authorGyöngyösi Adrienn (1982-) (biológus)
dc.contributor.submitterdepÉlettani Intézet -- 10
dc.contributor.submitterdepImmunológiai Intézet -- 18
dc.contributor.submitterdepMolekuláris Orvostudomány Doktori Iskola -- 10042
dc.contributor.submitterdepÁOK
dc.contributor.submitterdepOrvostudományi Doktori Tanács
dc.contributor.submitterdepDebreceni Egyetem
dc.date.accessioned2019-07-05T08:39:45Z
dc.date.available2019-07-05T08:39:45Z
dc.date.oa2019-07-08
dc.date.updated2019-07-05T08:39:45Z
dc.description.abstractNicotinic acid (NA) activates hydroxycarboxylic acid receptor 2 (HCA2), and it is widely used in treating dyslipidemias. Since its side effects include skin dryness, whereas its deficiency can be accompanied by dyssebacia, characterized by sebaceous gland enlargement, we asked if HCA2 is expressed on human sebocytes, and if NA influences sebocyte functions. By using human immortalized SZ95 sebocytes, we found that non-cytotoxic (≤100 μM; MTT-assay) concentrations of NA had no effect on the homeostatic sebaceous lipogenesis (SLG; Nile Red), but normalized excessive, acne-mimicking SLG induced by several lipogenic agents (arachidonic acid, anandamide, linoleic acid+testosterone; Nile Red; 48-hr treatments). Moreover, it exerted significant anti-proliferative actions (CyQUANT-assay), and increased [Ca2+]IC (Fluo-4 AM-based Ca2+-measurement). Although NA did not prevent the lipopolysaccharide-induced pro-inflammatory response (up-regulation [Q-PCR] and release [ELISA] of several pro-inflammatory cytokines) of the sebocytes, collectively, these data support the concept that NA may be effective in suppressing sebum production in vivo. While exploring the mechanism of the sebostatic actions, we found that sebocytes express HCA2 (Q-PCR, immunofluorescent labeling), siRNA-mediated silencing of which prevented the NA-induced Ca2+-signal and the lipostatic action. Collectively, our data introduce NA, and HCA2 activators in general, as novel, potent, and most likely safe sebostatic agents, with possible anti-acne potential.
dc.description.correctorKA
dc.identifier.citationJournal Of Cellular And Molecular Medicine. -[Epub] : - (2019), p. -. -J. Cell. Mol. Med. -1582-1838
dc.identifier.doi10.1111/jcmm.14505
dc.identifier.issn1582-1838
dc.identifier.opachttp://webpac.lib.unideb.hu:8082/ebib/CorvinaWeb?action=cclfind&resultview=long&ccltext=idno+BIBFORM079699
dc.identifier.urihttp://hdl.handle.net/2437/270273
dc.identifier.urlhttps://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.14505
dc.languageeng
dc.rights.accessopen access journal
dc.rights.ownerszerző
dc.subject.otheridegen nyelvű folyóiratközlemény külföldi lapban
dc.subject.otheracne
dc.subject.otherhydroxycarboxylic acid receptor 2
dc.subject.othernicotinic acid
dc.subject.othersebaceous lipogenesis
dc.subject.othersebocyte
dc.subject.otherseborrhea
dc.tenderGINOP-2.3.2-15-2016-00050 -- GINOP
dc.tender120552, 121360, 125055 -- OTKA
dc.tenderNKFIH NVKP_16‐1‐2016‐0042 -- Egyéb
dc.tenderEU's Horizon 2020 research and innovation program No. 739593 -- Egyéb
dc.tenderBolyai János Kutatási Ösztöndíj -- MTA
dc.tenderÚj Nemzeti Kiválóság Program ÚNKP‐18‐4‐DE‐247 -- Egyéb
dc.tenderNemzeti Tehetség Program NTPNFTÖ‐ 18‐B‐0168 -- Egyéb
dc.tenderGalderma International "Acne and rosacea basic research award 2015" -- Egyéb
dc.titleNicotinic acid suppresses sebaceous lipogenesis of human sebocytes via activating hydroxycarboxylic acid receptor 2 (HCA )
dc.typefolyóiratcikk
Fájlok
Eredeti köteg (ORIGINAL bundle)
Megjelenítve 1 - 2 (Összesen 2)
Nem elérhető
Név:
FILE_UP_1_24_Nicotinic acid suppresses sebaceous lipogenesis of human sebocytes via activating hydroxycarboxylic acid receptor 2 (HCA2) Markovics 2019.pdf
Méret:
1.05 MB
Formátum:
Adobe Portable Document Format
Leírás:
Kiadói változat
Letöltés
Nem elérhető
Név:
FILE_UP_2_24_Nicotinic acid suppresses sebaceous lipogenesis of human sebocytes via activating hydroxycarboxylic acid receptor 2 (HCA2) Markovics 2019 SD.pdf
Méret:
474.57 KB
Formátum:
Adobe Portable Document Format
Leírás:
supplementary material
Letöltés
Gyűjtemények
Publikációs Adatbázis