UV-irradiációt követő DNS-károsodás és reparáció a bőr sejtjeiben. In vitro vizsgálatok

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dc.contributor.advisorHorkay, Irén
dc.contributor.advisorRemenyik, Éva
dc.contributor.authorEmri, Gabriella
dc.contributor.departmentEgészségtudományok doktori iskolahu
dc.date.accessioned2007-07-19T12:18:50Z
dc.date.available2007-07-19T12:18:50Z
dc.date.defended2004-10-28
dc.description.abstractIn the present work we studied the DNA-damaging effect of solar UV-radiation at different biological endpoints after exposure. Our results demonstrate the usefulness of the comet-assay and the micronucleus (MN)-assay in this research field. 1. Using comet-assay we could differentiate between UVA- and UVB-irradiation induced DNA-damage and repair in cultured human HaCaT keratinocytes. Our results are in agreement with literature data. Furthermore, we have found that the alkaline comet-assay is not able to show the effect of PUVA (8-MOP+UVA) on DNA. 2. We could demonstrate by means of neutral comet-assay the formation of DNA-double strand breaks during DNA-repair following PUVA-induced DNA-damage. 3. A flow cytometry method we modified and applied made it possible to measure MN-induction with differentiation between nuclei in G0/G1- and G2/M-phase at the same time in cultured normal human fibroblasts (FB) ad keratinocytes (KC). We observed positive correlation between MN-induction and proportion of G2/M-phase nuclei in the sample. 4. We were the first to report that UVA is able to induce chromosomal damage in a dose dependent manner in human FB. 5. We have found that UVB-irradiation also induces chromosomal damage in a dose dependent manner in human melanocytes (MC) and FB, but the extent of the DNA-damage is different in the two cell types regarding the same radiation doses. 6. By means of comet-assay we have found that upon 4 h and 8 h of exposure to very low concentrations of formaldehyde (FA) significant DNA-protein crosslink-formation is present in normal human FB and in normal human KC. 7. We were the first to describe DNA-damage and repair kinetics induced by UV-irradiation in cultured normal human KC in comparison with FB using comet-assay. The DNA-damage and repair following UV-irradiation were dependent on the wavelength of UV and the repair kinetics was similar in the two cell types. 8. We have found that FA at a low concentration level interfered with DNA-resynthesis/ligation step of nucleotide excision repair following UVC- and UVB-irradiation. FA had no effect on the DNA-repair after UVA-irradiation. 9. We have found that the delay in DNA-repair resulted in an increase of chromosomal damage. FA at a concentration not inducing MN caused significant increase of UVC-induced chromosomal damage.en
dc.description.correctorde
dc.description.degreePhDhu_HU
dc.format.extent405245 bytes
dc.format.extent220595 bytes
dc.format.extent1055424 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/2437/2655
dc.language.isohuen
dc.language.isoenen
dc.subject.disciplineEgészségtudományokhu
dc.subject.sciencefieldOrvostudományokhu
dc.titleUV-irradiációt követő DNS-károsodás és reparáció a bőr sejtjeiben. In vitro vizsgálatoken
dc.title.translatedUV-Irradiation Induced DNA-Damage and Repair in Skin Cells. In Vitro Methodsen
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