Co-transformation of bean callus using high-velocity microprojectiles- mediated DNA transfer

A tétel rövid nézete
dc.creatorEissa Ahmed, E.
dc.creatorBisztray, Gy.
dc.creatorVelich, I.
dc.date2001-03-21
dc.date.accessioned2021-06-28T09:57:53Z
dc.date.available2021-06-28T09:57:53Z
dc.descriptionWe have found that 50 mg/I kanamycin and 0.8 Mo1/1 mannitol concentration was sufficient to kill the control callus of bean (Phaseolus vulgaris L.) and differentiate transgenic from the non-transgenic cells. The GeneBooster particle delivery system was used for the bombardment of bean callus. The kanamycin resistance gene was used as a selectable marker. The test was made by transferring the healthy white callus, subcultured for three months on selective and non-selective medium. After selection on kanamycin containing media, several kanamycin resistant calli had been obtained, survived and grew. After selection on mannitol containing media no drought resistant calli had been obtained. Resistance of the selected calli were verified by their ability to grow repeatedly on selective medium containing 150 mg/I kanamycin. Selective pressure was maintained over a period of 8 months.
dc.formatapplication/pdf
dc.identifierhttps://ojs.lib.unideb.hu/IJHS/article/view/253
dc.identifier10.31421/IJHS/7/1/253
dc.identifier.urihttp://hdl.handle.net/2437/314089
dc.languageeng
dc.publisherUniversity of Debrecen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/253/252
dc.sourceInternational Journal of Horticultural Science; Vol. 7 No. 1 (2001); 76-78.
dc.source2676-931X
dc.source1585-0404
dc.source10.31421/IJHS/7/1
dc.subjectPhaseolus vulgaris
dc.subjectcallus
dc.subjectGeneBooster
dc.subjectkanamycin resistance
dc.subjectmannitol
dc.subjecttransformation
dc.titleCo-transformation of bean callus using high-velocity microprojectiles- mediated DNA transfer
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
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International Journal of Horticultural Science