Micropropagation of Rudbeckia hirta L. from seedling explants

A tétel rövid nézete
dc.contributor.authorSzarvas, P.
dc.contributor.authorZsila-André, A.
dc.contributor.authorKováts, Z.
dc.contributor.authorFári, M. G.
dc.date.accessioned2021-06-28T09:59:23Z
dc.date.available2021-06-28T09:59:23Z
dc.date.issued2005-05-18
dc.description.abstractWe conducted experiments for developing an in vitro micropropagation protocol starting from meristems of Rudbeckia hirta L seedlings. We pre-soaked the seeds in sterile ion-exchanged water for 17 hours, and then achieved surface disinfection in two separate steps. First, we used concentrated household sodium-hypochloride solution for 20 minutes and, also for 20 minutes, we applied hydrogen peroxide of 10%, which was followed by washing with sterile ion-exchanged water three times. For the propagation of seedling meristems, the combination of half-strenght solid Murashige and Skoog (1962) culture medium containing 10 mg/1 of kinetin or 2 mg/I of kinetin + 0.1 mg/1 of 2iP proved to be the most suitable. The average number of shoot-buds developed from the seedling axillary meristem in the best culture media varied between 5 and 17. Without separating them, we inoculated the shoot-bud clusters on MS culture medium containing 2 mg/1 of IAA. After four weeks of incubation we obtained elongated shoots which we separated and inoculated into a new culture medium and we obtained elongated roots. The rooted plants were gradually acclimatised in the cultivation room, potted and carried to a greenhouse, and then planted in open field for subsequent observation. By adopting this method, our laboratory started the micropropagation of the superior and/or elite genotypes of the Rudbeckia hirta L. being of special value in respect of breeding.en
dc.formatapplication/pdf
dc.identifier.citationInternational Journal of Horticultural Science, Vol. 11 No. 2 (2005) , 105-108
dc.identifier.doihttps://doi.org/10.31421/IJHS/11/2/588
dc.identifier.eissn2676-931X
dc.identifier.issn1585-0404
dc.identifier.issue2
dc.identifier.jatitleInt. j. hortic. sci.
dc.identifier.jtitleInternational Journal of Horticultural Science
dc.identifier.urihttps://hdl.handle.net/2437/314325en
dc.identifier.volume11
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/588
dc.rights.accessOpen Access
dc.rights.ownerInternational Journal of Horticultural Science
dc.subjectRudbeckia hirta L.en
dc.subjectin vitroen
dc.subjecttissue cultureen
dc.subjectseedlingsen
dc.subjectmicropropagationen
dc.subjectHungaryen
dc.titleMicropropagation of Rudbeckia hirta L. from seedling explantsen
dc.typefolyóiratcikkhu
dc.typearticleen
dc.type.detailedidegen nyelvű folyóiratközlemény hazai lapbanhu
Fájlok
Eredeti köteg (ORIGINAL bundle)
Megjelenítve 1 - 1 (Összesen 1)
Nincs kép
Név:
pdf
Méret:
1.89 MB
Formátum:
Adobe Portable Document Format
Letöltés
Gyűjtemények
International Journal of Horticultural Science (DE-journals)