Micropropagation of Rudbeckia hirta L. from seedling explants

A tétel rövid nézete
dc.creatorSzarvas, P.
dc.creatorZsila-André, A.
dc.creatorKováts, Z.
dc.creatorFári, M. G.
dc.date2005-05-18
dc.date.accessioned2021-06-28T09:59:23Z
dc.date.available2021-06-28T09:59:23Z
dc.descriptionWe conducted experiments for developing an in vitro micropropagation protocol starting from meristems of Rudbeckia hirta L seedlings. We pre-soaked the seeds in sterile ion-exchanged water for 17 hours, and then achieved surface disinfection in two separate steps. First, we used concentrated household sodium-hypochloride solution for 20 minutes and, also for 20 minutes, we applied hydrogen peroxide of 10%, which was followed by washing with sterile ion-exchanged water three times. For the propagation of seedling meristems, the combination of half-strenght solid Murashige and Skoog (1962) culture medium containing 10 mg/1 of kinetin or 2 mg/I of kinetin + 0.1 mg/1 of 2iP proved to be the most suitable. The average number of shoot-buds developed from the seedling axillary meristem in the best culture media varied between 5 and 17. Without separating them, we inoculated the shoot-bud clusters on MS culture medium containing 2 mg/1 of IAA. After four weeks of incubation we obtained elongated shoots which we separated and inoculated into a new culture medium and we obtained elongated roots. The rooted plants were gradually acclimatised in the cultivation room, potted and carried to a greenhouse, and then planted in open field for subsequent observation. By adopting this method, our laboratory started the micropropagation of the superior and/or elite genotypes of the Rudbeckia hirta L. being of special value in respect of breeding.
dc.formatapplication/pdf
dc.identifierhttps://ojs.lib.unideb.hu/IJHS/article/view/588
dc.identifier10.31421/IJHS/11/2/588
dc.identifier.urihttp://hdl.handle.net/2437/314325
dc.languageeng
dc.publisherUniversity of Debrecen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/588/587
dc.rightsCopyright (c) 2018 International Journal of Horticultural Science
dc.sourceInternational Journal of Horticultural Science; Vol. 11 No. 2 (2005); 105-108
dc.source2676-931X
dc.source1585-0404
dc.source10.31421/IJHS/11/2
dc.subjectRudbeckia hirta L.
dc.subjectin vitro
dc.subjecttissue culture
dc.subjectseedlings
dc.subjectmicropropagation
dc.subjectHungary
dc.titleMicropropagation of Rudbeckia hirta L. from seedling explants
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
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International Journal of Horticultural Science