Effect of bile on hemodynamics and blood micro-rheological parameters in an experimental porcine model of bilemia

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dc.contributor.advisorDeák, Ádám
dc.contributor.advisorVarga, Ádám
dc.contributor.advisordeptÁltalános Orvostudományi Kar::Sebészeti Műtéttani Tanszék
dc.contributor.authorTran, Nghi Bao
dc.contributor.departmentDE--Általános Orvostudományi Kar
dc.contributor.opponentNghi Bao
dc.contributor.opponentdeptÁltalános Orvostudományi Kar
dc.date.accessioned2025-07-25T07:46:58Z
dc.date.available2025-07-25T07:46:58Z
dc.date.created2025
dc.description.abstractBilemia is characterized by the entering of bile into blood circulation, which can be caused by hepatic damage. Its hemodynamic and hemorheological effects have been investigated in mice and are yet to be revealed. Therefore, our study aimed to investigate hemodynamic and micro-rheological parameter changes in the porcine model via experimental bilemia in both in vivo and in vitro studies. Via median laparotomy, bile was collected from the gallbladder of eight anesthetized female swine. The blood sample was collected through the cannulated external jugular vein. Samples of 1000 μl anticoagulated blood were mixed with 1 μl, 5 μl and 20 μl bile for in vitro investigation. In vivo, the systemic effect was studied by administering bile intravenously (200 μl bile/kg) and closely monitoring heart rate and blood pressure through the cannulated left common carotid artery. Micro-rheological parameters were also tested, including hematological parameters, red blood cell (RBC) deformability, osmotic gradient deformability and RBC aggregation. In vivo, there was a decline in white blood cell and platelet count as well as a significant and temporary drop in blood pressure and heart rate (24.61% decrease compared to the based values). In vitro, there was a significant decrease in RBC aggregation parameters with increasing bile concentration (base vs. 20 μl bile: p<0.001). A dose-related change was observed with the bile concentration, as there was a decrease in the maximal elongation index and the SS1/2 parameter, which characterize the red blood cells’ deformability (base vs. 1 μl bile: p=0.033). Bilemia has clear and transient effects on hemodynamic parameters, leading to micro- rheological deterioration. In vitro experiments showed a potential dose-dependent effect. The direct effect of bile and biliary acids can cause microcirculatory and micro-rheological changes, which can result in liver damage complications.
dc.description.courseáltalános orvos
dc.description.courselangangol
dc.description.degreeegységes, osztatlan
dc.format.extent27
dc.identifier.urihttps://hdl.handle.net/2437/396128
dc.language.isoen
dc.rights.infoHozzáférhető a 2022 decemberi felsőoktatási törvénymódosítás értelmében.
dc.subjectbilemia
dc.subjectmicro-rheology
dc.subjectswine model
dc.subject.dspaceMedicine
dc.titleEffect of bile on hemodynamics and blood micro-rheological parameters in an experimental porcine model of bilemia
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