Comprehensive evaluation of plant extracts generated effects using multiple animal models for fish feed development
| dc.contributor.advisor | Máthé , Endre | |
| dc.contributor.author | Alaya, Amina | |
| dc.contributor.department | Állattenyésztési tudományok doktori iskola | hu |
| dc.contributor.submitterdep | Mezőgazdaság-, Élelmiszertudományi és Környezetgazdálkodási Kar | |
| dc.date.accessioned | 2025-01-09T06:44:38Z | |
| dc.date.available | 2025-01-09T06:44:38Z | |
| dc.date.defended | 2025-01-24 | |
| dc.date.issued | 2024 | |
| dc.description.abstract | The use of plant extracts in medicine spans the history of mankind, encompassing different cultures and civilizations. This tradition, enriched by the collective knowledge of civilizations such as the Christian, Chinese, Indian and Islamic worlds, has evolved into the holistic approach of phytotherapy. Distinct from orthodox pharmacology, phytotherapy focuses on utilizing whole plants to promote overall health, reflecting a resurgence in interest towards natural, less forceful therapeutic alternatives, especially given the limitations of synthetic drugs like adverse secondary effects or antibiotic resistance. This study integrates botany, chemistry, pharmacology, developmental biology, biochemistry, microbiology, and clinical sciences to investigate the medicinal properties of plant extracts, leveraging advanced analytical techniques such as NMR spectroscopy, GC-MS, and HPLC for identifying and quantifying bioactive compounds. Among these, polyphenols and flavonoids are of particular interest due to their potential for antidiabetic, anti-inflammatory, antioxidant, and neuroprotective activities, which are crucial in preventing and managing chronic diseases and in cell metabolism processes. The research specifically targets the biological effects of olive (Olea europaea), sweet almond (Prunus amygdalus), and black mulberry (Morus nigra) species, known for their health-promoting properties. Olive products, for instance, are rich in antioxidants; almonds are appreciated for their lipid-lowering and anti-stress effects; and black mulberries are noted for their antihypertensive, anti-diabetic, and anti-inflammatory properties. The objective is to elucidate the corresponding GTEs nutritive and viability influencing effects, cellular mechanisms, and induction by these plant extracts through a multi-disciplinary approach, assessing their potential antimicrobial activity and contribution to nutrition and metabolism. The preparation of GTEs involved collecting young olive shoots, sweet almond buds, and black mulberry buds, with a strict quality control process to ensure high-quality extracts. These were prepared using a mixture of 96% ethanol and glycerol at a 1:1 ratio, ensuring the preservation of the plant materials' natural qualities and therapeutic potentials. Quantitative analysis was performed using the Shimadzu Nexera I LC-MS-8045 system, highlighting the meticulous process involved in analyzing the protein and carbohydrate contents through the Kjeldahl method and the phenol–sulfuric acid method, respectively. The antimicrobial properties of the GTEs were investigated against a selection of bacteria and fungi, revealing varying degrees of effectiveness. For instance, olive GTE showed significant activity against five microbial strains, particularly effective within a concentration range of 50-100%. The study also explored the antimicrobial effects of GTEs using the agar diffusion method, with the olive GTE demonstrating effectiveness against specific strains at concentrations as low as 50 mg/mL. Experiments on Drosophila melanogaster and carp larvae aimed to assess the GTEs effecting growth, viability, and metabolic health under different dietary conditions. In Drosophila, the HS diet caused a developmental delay, but GTE supplementation showed variable effects on larval viability without altering the delay. Olive-GTE notably improved viability in NM diets and to a lesser extent in HS diets, displaying a concentration-dependent impact. Similarly, Black Mulberry GTE demonstrated a significant increase in survival rates up to 26%, showcasing the nuanced differences in nutritional impacts of various GTEs on fruit fly development and survival. The qualitative and quantitative chemical analyses revealed unique phytonutrient profiles for each GTE, identifying bioactive compounds such as hydroxytyrosol, oleuropein in O-GTE, and unique fatty acids in SA-GTE. These analyses highlighted the rich diversity of compounds within the GTEs and their potential health benefits, suggesting their utility in health promotion and disease prevention. The study's findings, particularly on the antimicrobial capabilities and the nutritional effects of GTEs on model organisms, underline the complex interplay between diet, nutritional content, and health. The dual-species approach enhances understanding of the GTEs' s associated nutritive effects, emphasizing the potential of these plant extracts in developing natural health solutions and contributing to nutritional science and phytotherapy fields. | |
| dc.format.extent | 161 | |
| dc.identifier.uri | https://hdl.handle.net/2437/384666 | |
| dc.language.iso | en | |
| dc.subject | Olea europaea, Prunus amygdalus, Morus nigra, Drosophila melanogaster, Cyprinus carpio, gemmotherapy extract, antihyperglycemic effect, antimicrobial activity, antifungal activity, anti-inflammatory, polyphenol, flavonoid | |
| dc.subject.discipline | Állattenyésztési tudományok | hu |
| dc.subject.sciencefield | Agrártudományok | hu |
| dc.title | Comprehensive evaluation of plant extracts generated effects using multiple animal models for fish feed development | |
| dc.title.translated | Comprehensive evaluation of plant extracts generated effects using multiple animal models for fish feed development | |
| dc.type | PhD, doktori értekezés | hu |
Fájlok
Engedélyek köteg
1 - 1 (Összesen 1)
Nincs kép
- Név:
- license.txt
- Méret:
- 1.93 KB
- Formátum:
- Item-specific license agreed upon to submission
- Leírás: