Authors studied the effect of different temperatures prior to fruit body appearance on the yield of 3 Pleurotus strains P70, HK35 and 357. After 17 days of incubation at 25 °C and 28 °C the substrate blocks were kept for 7 days at 13, 16, 19, 22 and 25 °C. Then temperature was reduces to 16 °C during harvest time. Tests showed difference of some degrees in the optimal prefruiting temperature requirements of the strains which could result in even in 10-15% surplus yield. It is advisable to use technologies specially adapted to strains.