Expression, purification and investigation of retrotransposon-derived PEG10 protein

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dc.contributor.advisorMótyán, János András
dc.contributor.advisordeptDepartment of Biochemistry and Molecular Biologyhu_HU
dc.contributor.authorVadadokhau, Uladzislau
dc.contributor.departmentDE--Általános Orvostudományi Karhu_HU
dc.contributor.opponentFarkas, Ilona
dc.contributor.opponentdeptDebreceni Egyetem::Általános Orvostudományi Kar::Orvosi Vegytani Intézethu_HU
dc.date.accessioned2019-06-21T09:34:24Z
dc.date.available2019-06-21T09:34:24Z
dc.date.created2019-04-15
dc.description.abstractDespite the fact that PEG10 was shown to be involved in placenta, adipose tissue, and cancer formation, it is little known about this protein at a molecular level. Especially, no solid data has been published regarding a retroviral-type aspartic protease of PEG10 so far. Autoproteolytic assay performed in this project implies that PEG10 aspartyl protease is active. Analysis of protease activity became possible due to the expression of the protein in the bacterial system we designed. Preliminary results show that PEG10 may cleave itself at some sites with the formation of cleavage products having different size. The structure and the role of these products are still unrevealed. In addition, the sensitivity of PEG10 aspartic protease to retroviral protease inhibitor makes the design of new inhibitors against PEG10 a striking prospect. Therefore, future research may be aimed to elucidate the role of cleavage products in the PEG10 function with further biochemical characterization of PEG10 activity. Results presented in this work will facilitate the characterization of PEG10 aspartic protease and the role of PEG10 in human biology.hu_HU
dc.description.coursemolekuláris biológiahu_HU
dc.description.courselangangolhu_HU
dc.description.coursespecBiokémia-genomikahu_HU
dc.description.degreeMSc/MAhu_HU
dc.format.extent41hu_HU
dc.identifier.urihttp://hdl.handle.net/2437/269915
dc.language.isoenhu_HU
dc.subjectRetrotransposon-derived proteinhu_HU
dc.subjectPEG10hu_HU
dc.subject-1 ribosomal frameshiftinghu_HU
dc.subjectprotein expression in bacteriahu_HU
dc.subjectaffinity chromatographyhu_HU
dc.subjectautoproteolysishu_HU
dc.subjectRetroviral aspartic proteasehu_HU
dc.subjectinhibition assayhu_HU
dc.subject.dspaceDEENK Témalista::Biológiai tudományokhu_HU
dc.titleExpression, purification and investigation of retrotransposon-derived PEG10 proteinhu_HU
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