The in vitro antifungal activity of rezafungin, a second-generation echinocandin, was compared to other commonly-used antifungals. The inhibitory activity of antifungals against common and rare Candida species was tested using broth microdilution method. Compared to caspofungin, anidulafungin, micafungin, amphotericin B and fluconazole, rezafungin showed excellent activity against common and rare Candida species. In particular, rezafungin showed promising activity against the emerging multidrug resistant pathogen Candida auris.