Detection of DNA mutations by PCR-TTGE method

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dc.contributor.authorCsikós, Ádám
dc.contributor.authorSimon, Ádám
dc.contributor.authorTisza, Ákos
dc.contributor.authorGulyás, Gabriella
dc.contributor.authorJávor, András
dc.contributor.authorCzeglédi, Levente
dc.date.accessioned2021-06-28T10:54:06Z
dc.date.available2021-06-28T10:54:06Z
dc.date.issued2014-03-20
dc.description.abstractIn our study PCR-temporal temperature gelelectrophoresis (TTGE) and MeltINGENY bioinformatic program were used to analyse the mutations in the genes of melanocortin-1 receptor (MC1R) and pituitary adenylate-cyclase activating polypeptide (PACAP) in cattle. Amplification of target DNA by PCR was performed with GC-clamp primers and non-GC-clamp primers in simplex PCR reactions. The fragments were separated by denaturing polyacrylamide gelelectrophoresis (denaturing agents: high temperature, urea) after PCR reactions.MC1R homozygous individuals were used for the reaction. We concluded that MeltINGENY program makes the decision and detection system easier, and more simple as the melting profile of target sequence is determined by the software. In case of MC1R gene, PCR-TTGE method is appropriate for SNP detection, however PACAP gene polymorphism can not be identified by the method, because PACAP mutations are not included in melting domains, therefore PCR-TTGE cannot detect them.en
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dc.identifier.citationActa Agraria Debreceniensis, No. 57 (2014) , 21-25
dc.identifier.doihttps://doi.org/10.34101/actaagrar/57/1954
dc.identifier.issn2416-1640
dc.identifier.issue57
dc.identifier.jatitleActa agrar. Debr.
dc.identifier.jtitleActa Agraria Debreceniensis
dc.identifier.urihttps://hdl.handle.net/2437/315827en
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/actaagrar/article/view/1954
dc.rights.accessOpen Access
dc.subjectcattleen
dc.subjectDNA mutationen
dc.subjectMC1Ren
dc.subjectPACAPen
dc.titleDetection of DNA mutations by PCR-TTGE methoden
dc.typefolyóiratcikkhu
dc.typearticleen
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