Selection of powdery mildew resistant and susceptible grapevine genotypes with molecular markers

A tétel rövid nézete
dc.creatorMolnár, Stella
dc.creatorGalbács, Zsuzsanna
dc.creatorHalász, Gábor
dc.creatorHoffmann, Sarolta
dc.creatorVeres, Anikó
dc.creatorSzőke, Antal
dc.creatorGalli, Zsolt
dc.creatorSzádeczky-Kardoss, Bence
dc.creatorKozma, Pál
dc.creatorKiss, Erzsébet
dc.creatorHeszky, László
dc.date2007-11-15
dc.date.accessioned2021-06-28T10:59:02Z
dc.date.available2021-06-28T10:59:02Z
dc.descriptionIncorporation of competitive quality and resistance against the most important fungal diseases (powdery and downy mildew) in a cultivar is one of the most important aims of grapevine breeding. In the 20th century, the most advanced results in grapevine resistance breeding were achieved by French researchers. They used resistant cultivars in more than 30% of their growing areas. In these varieties, North American wild Vitisspecies were the resistance gene sources. The discovery of immunity-like resistance of Muscadinia rotundifolia opened new perspectives in resistance breeding. M. rotundifolia harbours a dominant powdery mildew gene, providing resistance in highquality cultivars after back-crosses with V. vinifera varieties. M. rotundifolia has been involved in the Hungarian grape breeding programs since 1996, thanks to a French-Hungarian variety exchange. In addition to traditional selection methods, application of MAS (Marker Assisted Selection) based on various types ofmolecular markers, can provide additional tools for these efforts. Run1 locus, responsible for powdery mildew resistance, was identified in Muscadinia rotundifolia. Molecular markers closely linked to this locus are very significant in screening progenies deriving from M. rotundifolia and V. vinifera crosses, making possible the discrimination between resistant and susceptible genotypes at DNA level. In our analyses BC5 progeny of {(M. rotundifola×V. vinifera) BC4}×Cardinal (V. vinifera) tested for powdery symptoms were analysed with PCR-RFLP (GLP1- 12P1P3) and microsatellite markers (VMC4f3.1, VMC8g9). Our results proved the applicability of the linked markers and reliability of marker assisted selection.
dc.formatapplication/pdf
dc.identifierhttps://ojs.lib.unideb.hu/actaagrar/article/view/3110
dc.identifier10.34101/actaagrar/27/3110
dc.identifier.urihttp://hdl.handle.net/2437/316630
dc.languageeng
dc.publisherFaculty of Agricultural and Food Sciences and Enviromental Management of the Debrecen University, Debrecen.
dc.relationhttps://ojs.lib.unideb.hu/actaagrar/article/view/3110/3079
dc.sourceActa Agraria Debreceniensis; No. 27 (2007); 100-104
dc.source2416-1640
dc.source1587-1282
dc.subjectpowdery mildew
dc.subjectVitis vinifera L.
dc.subjectMuscadinia rotundifolia
dc.subjectRun1 gene PCR-RFLP
dc.subjectmicrosatellite
dc.subjectmolecular marker
dc.titleSelection of powdery mildew resistant and susceptible grapevine genotypes with molecular markers
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Article
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Acta Agraria Debreceniensis