Study of miRNA expression in invasive aspergillosis

A tétel rövid nézete
dc.contributor.advisorPaholcsek, Melinda
dc.contributor.advisorTolnai, Emese
dc.contributor.advisordeptHuman Geneticshu_HU
dc.contributor.advisordeptDebreceni Egyetem::Általános Orvostudományi Karhu_HU
dc.contributor.authorNwozor, Kingsley Okechukwu
dc.contributor.departmentDE--Általános Orvostudományi Karhu_HU
dc.contributor.opponentScholtz, Beáta
dc.contributor.opponentdeptDebreceni Egyetem, Általános Orvostudományi Karhu_HU
dc.contributor.opponentdeptBiokémiai és Molekuláris Biológiai Intézethu_HU
dc.date.accessioned2018-06-22T09:22:26Z
dc.date.available2018-06-22T09:22:26Z
dc.date.created2018-06-19
dc.description.abstractIntroduction: miRNAs are key regulators of various physiological and pathophysiological processes and their altered expression levels have been associated to many diseases. Increasing number of evidences have demonstrated that specific miRNAs can serve as novel biomarkers for clinical diagnosis of diseases. Invasive aspergillosis is life-threatening systemic fungal infection. Regrettably, the standard diagnostic methods for this condition take days to produce a reliable result. Objectives: The aim of this study was to probe the expression profiles of 15 selected miRNAs in patients, with fevered neutropenia. We hypothesized that invasive aspergillosis could change the miRNA profiles in the blood. Our goal was to make a rapid test supporting diagnosis of invasive mycoses. Methods: We obtained whole blood samples from 16 patients suffering neutropenic fever and from 5 healthy volunteers without neutropenic fever. Total RNA was isolated from the whole blood and were reverse transcribed to cDNA. TaqMan® quantitative real-time PCR (miRNA assay) was used for detecting miRNA expression profiles. Relative mRNA expressions were calculated with the ΔΔCt method. Results: 7 out of the 19 miRNAs showed significant upregulation in the expression levels at p<0.05. Conclusion: The miRNAs with significantly different expression between the test group and the control can be used as informative biomarkers to assess and monitor the body’s physio-pathological status in invasive aspergillosis.hu_HU
dc.description.coursemolekuláris biológiahu_HU
dc.description.courselangangolhu_HU
dc.description.coursespecBiokémia-genomikahu_HU
dc.description.degreeMSc/MAhu_HU
dc.format.extent31hu_HU
dc.identifier.urihttp://hdl.handle.net/2437/255248
dc.language.isoenhu_HU
dc.subjectNeutropeniahu_HU
dc.subjectAspergillosishu_HU
dc.subjectmicroRNAhu_HU
dc.subjectBiomakershu_HU
dc.subject.dspaceDEENK Témalista::Biológiai tudományokhu_HU
dc.titleStudy of miRNA expression in invasive aspergillosishu_HU
Fájlok
Gyűjtemények
Theses (Faculty of Medicine)