Ultrastructural and biochemical aspects of normal and hyperhydric eucalypt

A tétel rövid nézete
dc.contributor.authorPicoli, E. A. T.
dc.contributor.authorPaiva, E. A. S.
dc.contributor.authorXavier, A.
dc.contributor.authorAguiar, R. M.
dc.contributor.authorCarolino, S. M. B.
dc.contributor.authorFári, M. G.
dc.contributor.authorOtoni, W. C.
dc.date.accessioned2021-06-28T10:00:43Z
dc.date.available2021-06-28T10:00:43Z
dc.date.issued2008-05-19
dc.description.abstractHyperhydricity was observed throughout in vitro multiplication phase of a Eucalyptus grandis clone. Ultrastructural approach of tissue and cell differentiation, izoenzyme patterns, binding protein (BiP) expression, and pigment content were performed. Hyperhydric tissues showed a reduction in cell wall deposition, reduction of membranous organelles, higher cell vacuolation, and more intercellular spaces than its normal counterpart. Additionally, several vesicles were present in hyperhydric cells suggesting the occurrence of organelle autophagy by autophagic vacuole. Lower pigment content, intercellular spaces on the epidermis and the induction of a molecular chaperone (BiP) were observed in hyperhydric phenotype. Evidences of schizolysigenous process of intercellular space formation are compatible with a stress condition. Although plastoglobulli were observed in normal and hyperhydric chloroplasts, they were more evident in the normal ones. Abnormal stomata also reflected a disruptive situation and morphogenesis disturbances which would difficult plant acclimatization. Further observation of the epidermis ultrastructure allows us to conclude that the presence of intercellular spaces on its surface may be constraining the recovery and development of hyperhydric plants. Similarly to BiP, other proteins such as esterase (EST), acid phosphatase (ACP), malate dehydrogenase (MDH) and peroxidase (PDX) are possible to be used as stress markers in in vitro conditions. Our results confirm earlier findings about negative effects of hyperhydricity on in vitro plant morphogenesis and ultrastructure, which in eucalypt is associated with a stressful condition contributing to lower propagation ratios.en
dc.formatapplication/pdf
dc.identifier.citationInternational Journal of Horticultural Science, Vol. 14 No. 3 (2008) , 61-69.
dc.identifier.doihttps://doi.org/10.31421/IJHS/14/3/805
dc.identifier.eissn2676-931X
dc.identifier.issn1585-0404
dc.identifier.issue3
dc.identifier.jatitleInt. j. hortic. sci.
dc.identifier.jtitleInternational Journal of Horticultural Science
dc.identifier.urihttps://hdl.handle.net/2437/314514en
dc.identifier.volume14
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/805
dc.rights.accessOpen Access
dc.rights.ownerInternational Journal of Horticultural Science
dc.subjectBiPen
dc.subjecthyperdrydricityen
dc.subjectisoenzymesen
dc.subjectmicropropagationen
dc.subjectpigmenten
dc.subjectstressen
dc.titleUltrastructural and biochemical aspects of normal and hyperhydric eucalypten
dc.typefolyóiratcikkhu
dc.typearticleen
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International Journal of Horticultural Science (DE-journals)