The function of dendritic cells (DCs) is to process antigen material and present it to T lymphocytes, initiating the adaptive immune response. DCs can be used in cell therapy to treat tumors or autoimmune diseases thus, they are an aspect when it comes to immunotherapy. In this study we tried to generate bone marrow derived DC (BM-DCs) and the major goal was to test various cytokines to get functional immune cells from adult stem cells. To produce BM-DCs, isolated mouse bone marrow cells were ex vivo differentiated for 9 days. The differentiated cells were cultured in the RPMI medium in the presence of GM-CSF, with or without IL-4 and/or Flt3L. Finally, LPS was added at day 8 to induce maturation and the obtained BM-DCs were harvested at day 9. We carried out multicolor flow cytometric analysis at day 9 to assess the expression of several DC and maturation specific proteins in BM-DCs. Our data revealed that GM-CSF combined with IL-4 leads to the formation of more activated BMDCs which are exhibited an elevated expression of MHC II, CD86, and CD40, especially, in the presence of LPS activation. In addition, our data suggest that high concentration of Flt3L modifies the phenotype of the GM-CSF instructed BM-DCs.