Linseed oil supplementation affects fatty acid desaturase 2, peroxisome proliferator activated receptor gamma, and insulin-like growth factor 1 gene expression in turkeys (Meleagris gallopavo)

A tétel rövid nézete
dc.contributor.authorSzalai, Klaudia
dc.contributor.authorTempfli, Károly
dc.contributor.authorZsédely, Eszter
dc.contributor.authorLakatos, Erika
dc.contributor.authorGáspárdy, András
dc.contributor.authorBali Papp, Ágnes
dc.contributor.statusPhD hallgatóhu_HU
dc.contributor.statusegyetemi oktató, kutatóhu_HU
dc.coverage.temporal2022.04.30.hu_HU
dc.date.accessioned2022-01-25T13:01:29Z
dc.date.available2022-01-25T13:01:29Z
dc.description.abstractObjective: Effects of linseed oil (LO) supplementation on the fat content and fatty acid profile of breast meat, and the expression of three genes in the liver, breast muscle and fat tissues of commercial 154-day-old hybrid male turkeys were investigated. Methods: The animals in the control group were fed a commercially available feed and received no LO supplementation (n = 70), whereas animals in the LO group (n = 70) were fed the same basic diet supplemented with LO (day 15 to 21, 0.5%; day 22 to 112, 1%). The effect of dietary LO supplementation on fatty acid composition of breast muscle was examined by gas chromatography, and the expression of fatty acid desaturase 2 (FADS2), peroxisome proliferator activated receptor gamma (PPAR gamma), and insulin-like growth factor 1 (IGF1) genes was analysed by means of quantitative reverse transcription polymerase chain reaction. Results: The LO supplementation affected the fatty acid composition of breast muscle. Hepatic FADS2 levels were considerably lower (p<0.001), while adipose tissue expression was higher (p<0.05) in the control compared to the LO group. The PPAR gamma expression was lower (p<0.05), whereas IGF1 was higher (p<0.05) in the fat of control animals. There were no significant (p>0.05) differences in FADS2, PPAR gamma, and IGF1 gene expressions of breast muscle; however, omega-6/omega-3 ratio of breast muscle substantially decreased (p<0.001) in the LO group compared to control. Conclusion: Fatty acid composition of breast meat was positively influenced by LO supplementation without deterioration of fattening parameters. Remarkably, increased FADS2 expression in the liver of LO supplemented animals was associated with a significantly decreased omega-6/omega-3 ratio, providing a potentially healthier meat product for human consumption. Increased PPAR gamma expression in fat tissue of the LO group was not associated with fat content of muscle, whereas a decreased IGF1 expression in fat tissue was associated with a trend of decreasing fat content in muscle of the experimental LO group.hu_HU
dc.format.extent662-669hu_HU
dc.identifier.urihttp://hdl.handle.net/2437/328159
dc.language.isoenhu_HU
dc.subjectFatty Acid Desaturase 2 (FADS2)hu_HU
dc.subjectGene Expressionhu_HU
dc.subjectInsulin-like Growth Factor 1 (IGF1)hu_HU
dc.subjectMeleagris gallopavohu_HU
dc.subjectPeroxisome Proliferator Activated Receptor Gamma (PPAR gamma)hu_HU
dc.subject.disciplinetudományterületek::állattenyésztési tudományokhu_HU
dc.titleLinseed oil supplementation affects fatty acid desaturase 2, peroxisome proliferator activated receptor gamma, and insulin-like growth factor 1 gene expression in turkeys (Meleagris gallopavo)hu_HU
dc.typeidegen nyelvű peer review publikációhu_HU
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Wittmann Antal Növény-, Állat- és Élelmiszertudományi DI