Macrophomina phaseolina is a polyphagous fungus that infects a wide array of more than 700 plant species. The disease belongs to soil-dwelling fungi, and origins from subtropical to tropical regions of the world. However, this pathogen can be found worldwide, and ranked on the top devastating diseases that could occur. On this study, we reviewed the morphological, genetical, epidemiology, and survivability characteristics of the fungus. While implementing a distinctive attempt to mitigate the development and growth of microsclerotia in vitro. Moreover, we covered the primary issues regarding the crucial circumstances and factors related to the microsclerotia production, and how it affects its growth rate and aggregation.