Vitamin D status is influenced by well-known deter-minants, but factors associated with low 25-hydroxy-vitamin D levels in the cutaneous melanoma popula-tion are not well defined. The aim of this study was to confirm the well-known determinants and to assess new determinants for 25-hydroxyvitamin D levels in a cutaneous melanoma population. In a prospectively included cohort of 387 patients with cutaneous mela-noma the association of 25-hydroxyvitamin D levels with sex, age, body mass index, time of blood with-drawal, Fitzpatrick phototype, vitamin D supplemen-tation, score for intensity of lifetime sun exposure, smok ing, education level, hair and skin colour, eye colour, total number of benign naevi, freckles and pa-rameters of chronic sun damage was investigated. In addition, 25-hydroxyvitamin D levels were correlated with pathological parameters of the primary tumour and melanoma stage (8th edition of the American Joint Committee on Cancer (AJCC). Univariate and multi-variate logistic regressions were performed using R software. The following factors had a significant effect on vitamin D status: body mass index, seasonal time of blood sampling, vitamin D supplementation, and a subtype of skin, and hair colour.