Detection of Target-Site Herbicide Resistance in the Common Ragweed: Nucleotide Polymorphism Genotyping by Targeted Amplicon Sequencing

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dc.contributor.authorKutasy, Barbara
dc.contributor.authorFarkas, Zoltán
dc.contributor.authorKolics, Balázs
dc.contributor.authorDecsi, Kincső
dc.contributor.authorHegedűs, Géza
dc.contributor.authorKovács, Judit
dc.contributor.authorTaller, János
dc.contributor.authorTóth, Zoltán
dc.contributor.authorKálmán, Nikoletta
dc.contributor.authorKazinczi, Gabriella
dc.contributor.authorVirág, Eszter
dc.contributor.statusPhD hallgatóhu_HU
dc.contributor.statusegyetemi oktató, kutatóhu_HU
dc.coverage.temporal2020.04.30.hu_HU
dc.date.accessioned2021-06-16T13:22:04Z
dc.date.available2021-06-16T13:22:04Z
dc.description.abstractBackground: The spread of herbicide-resistance Ambrosia artemisiifolia threatens not only the production of agricultural crops, but also the composition of weed communities. The reduction of their spread would positively affect the biodiversity and beneficial weed communities in the arable habitats. Detection of resistant populations would help to reduce herbicide exposure which may contribute to the development of sustainable agroecosystems. Methods: This study focuses on the application of target-site resistance (TSR) diagnostic of A. artemisiifolia caused by different herbicides. We used targeted amplicon sequencing (TAS) on Illumina Miseq platform to detect amino acid changes in herbicide target enzymes of resistant and wild-type plants. Results: 16 mutation points of four enzymes targeted by four herbicide groups, such as Photosystem II (PSII), Acetohydroxyacid synthase (AHAS), 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) and protoporphyrinogen IX oxidase (PPO) inhibitors have been identified in common ragweed populations, so far. All the 16 mutation points were analyzed and identified. Out of these, two mutations were detected in resistant biotypes. Conclusions: The applied next-generation sequencing-targeted amplicon sequencing (NGS-TAS) method on A. artemisiifolia resistant and wild-type populations enable TSR detection of large sample numbers in a single reaction. The NGS-TAS provides information about the evolved herbicide resistance that supports the integrated weed control through the reduction of herbicide exposure which may preserve ecological properties in agroecosystems.hu_HU
dc.format.extent16hu_HU
dc.identifier.urihttp://hdl.handle.net/2437/311190
dc.language.isoenhu_HU
dc.subjecttarget-site herbicide resistancehu_HU
dc.subjectahashu_HU
dc.subjectalshu_HU
dc.subjectpsbAhu_HU
dc.subjectepspshu_HU
dc.subjecttargeted amplicon sequencinghu_HU
dc.subjectAmbrosia artemisiifoliahu_HU
dc.subject.disciplinetudományterületek::növénytudományokhu_HU
dc.titleDetection of Target-Site Herbicide Resistance in the Common Ragweed: Nucleotide Polymorphism Genotyping by Targeted Amplicon Sequencinghu_HU
dc.typeidegen nyelvű peer review publikációhu_HU
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