The neurons in the spinocerebellar and pontocerebellar circuits share a common birthday

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dc.contributor.advisorMészár, Zoltán
dc.contributor.advisordeptÁltalános Orvostudományi Kar::Anatómiai, Szövet- és Fejlődéstani Intézet
dc.contributor.authorTodd, Alistair
dc.contributor.departmentDE--Általános Orvostudományi Kar
dc.date.accessioned2025-05-11T09:40:26Z
dc.date.available2025-05-11T09:40:26Z
dc.date.created2025-03-17
dc.description.abstractThe development of the vertebrate hindbrain is controlled by hox genes, which work together to create a precise schedule for the differentiation of neurons. This is essential for the assembly of functional circuits. We hypothesized that neurons born in different regions of the central nervous system at the same time preferentially connect to form functional circuits (same-born-same-circuit theory). Previously, we found that the latest neurons born in the spinal dorsal horn were those that populate the posterior thoracic nucleus (nucleus of Clarke) in lamina VII. These neurons project axons through the spinocerebellar tract, which forms the mossy fibers and terminates in the granular layer of the cerebellar cortex. We aimed to learn more about the developmental schedule of the neurons that provide inputs to the cerebellum by mapping those neurons born at the same time as the posterior thoracic nucleus. To label neurons born at the same time, we injected a GFP coding DNA expression vector into the fourth ventricle of 12.5-day old mouse embryos. We transfected the newborn neurons with in utero electroporation technique and allowed the embryos to grow and be born. We then let the mice reach young adult age (P28), and after that, we sacrificed and fixed them by transcranial perfusion with formaldehyde. We dissected their brains with the spinal cord and selected them based on successful GFP labeling. We made serial sections of the hindbrain regions, and the GFP signal was enhanced with immunohistochemistry. The histochemical reaction was visualized with diaminobenzidine, and the sections were mounted on histological slides. In the spinal cord, we found dense labeling in the Clarke’s nucleus, as well as in the superficial spinal dorsal horn. The labeling in the deep lamina was more prominent among the astrocytes, indicating that neurogenesis is followed by gliogenesis in a temporally overlapping manner. We also found labeling in the reticular nuclei and vestibular nuclei of the brainstem. However, we did not find labeled cerebellar granule cells, but we did find dense labeling of the cerebellar glomeruli. We only found scattered labeling among the Purkinje cells, and we also labeled Golgi cells in some cases. Our results support our same-born-same-circuit theory but remain controversial due to the methods utilized.
dc.description.courseáltalános orvos
dc.description.courselangangol
dc.description.degreeegységes, osztatlan
dc.format.extent53
dc.identifier.urihttps://hdl.handle.net/2437/389788
dc.language.isoen
dc.rights.infoHozzáférhető a 2022 decemberi felsőoktatási törvénymódosítás értelmében.
dc.subjectNeural Tube
dc.subjectNeurohistogenesis
dc.subjectBrainstem
dc.subjectCerebellum
dc.subjectClarke’s Nucleus
dc.subjectSpinocerebellar Tract
dc.subject.dspaceBiology::Neurobiology
dc.titleThe neurons in the spinocerebellar and pontocerebellar circuits share a common birthday
dc.title.translatedA spinocerebelláris és pontocerebelláris pályák neuronjai közös időpontban keletkeznek
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