The research aim is to study esterase production and the effect of heat treatment on the esterase activity of LAB and also to test LAB esterase for zearalenone degradation. This experiment will provide data on the impact of temperature on the esterase activity of the mentioned LAB strains. This experiment's results can also be used as a reference for the next step to determine more effective usage of LAB species, especially with high esterase activity as a zearalenone degradation agent.