Unconventional T cells in health and diseases

A tétel rövid nézete
dc.contributor.advisorHevessy, Zsuzsanna
dc.contributor.authorSingh, Parvind
dc.contributor.authorvariantSingh, Parvind
dc.contributor.departmentLaki Kálmán doktori iskolahu
dc.contributor.submitterdepÁltalános Orvostudományi Kar::Laboratóriumi Medicina Intézet
dc.date.accessioned2024-10-22T12:40:32Z
dc.date.available2024-10-22T12:40:32Z
dc.date.created2024
dc.date.defended2024-11-07
dc.description.abstractTo summarize, the frequency of γδ and MAIT cells is higher in the younger age group, revealing a negative correlation with age and supporting the concept of immunosenescence. In contrast, iNKT cell frequency shows no statistically significant difference across age groups and no observable negative correlation with age. Gender-specific analysis uncovers higher levels of iNKT cells in females, exhibiting a negative correlation with age. In comparison, γδ T cells display higher levels in males, with both genders negatively correlating with age. MAIT cells, on the other hand, exhibit no gender-based differences and manifest a negative correlation with age in both males and females. The intriguing discovery of a positive correlation among uT cells lays a foundation for future studies exploring immune-mediated diseases and their relationship with uT cells. In vitro study revealed that MAIT cells were producing IL-13 in steady-state conditions and responded robustly to the 5-OP-RU ligand, specifically in the presence of proinflammatory cytokines (IL-12/33). Our in vitro experiments showed that MAIT cells regulate the surface expression of CD69 and CD38 and produce IFNγ and effector (TNFα/GzB) cytokines. We found a similar pattern of cell surface markers and cytokines in AD. This phenotype may correspond to an activated and fully differentiated effector cell type capable of producing cytokines and acting as a cytotoxic cell, suggesting their involvement in the pathomechanism of the disease. Cyclosporin A is a potent immunosuppressing agent that significantly blocks the proinflammatory cytokines in MAIT cells, while vitamin D3 could support MAIT cell activation and IL-13 production to bring steady-state conditions. Despite sharing the Vα7.2 compartment, MAIT cells and Vα7.2+/CD161- T cells show clear differences. Vα7.2+/CD161- T cells resemble conventional T cells and do not respond to 5-OP-RU, although PMA stimulation increases the production of certain cytokines.
dc.format.extent122
dc.identifier.urihttps://hdl.handle.net/2437/381325
dc.language.isoen
dc.subjectUnconventional T cells; iNKT cells; γδ T cells; MAIT cells; Vα7.2+/CD161- T cells; Atopic dermatitis; Immunosenescence; Reference range; Cyclosporin A; Vitamin D; t-SNE; UMAP; FlowSOM; Multidimensional data analysis; Age-dependent; Frequency; Gender dependency; Flow cytometry; Dimensionality reduction; Unsupervised clustering; Stimulation; Immunomodulation
dc.subject.disciplineKlinikai orvostudományok
dc.subject.sciencefieldOrvostudományokhu
dc.titleUnconventional T cells in health and diseases
dc.title.translatedNem-konvencionális T sejtek vizsgálata egészségesekben és betegekben
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