INTRODUCTION: In case of vascular surgeries appropriate regeneration of the vessel is a crucial part of the patient’s recovery. One way to increase the regeneration is to avoid inflammation because the inflammatory processes can be damaging to the tissues. The pituitary adenylate cyclase-activating polypeptide (PACAP) is a molecule with a proven antiapoptotic effect. In case of therapy the PACAP is absorbed and it can have a systemic effect besides of the local one. Bioplasts can also be used to assist vascular anastomosis healing. The aim of this study is to examine the systemic effect of the molecule and the bioplast on the hemorheological and microcirculatory parameters. MATERIALS AND METHODS: In this experiment (permission registration Nr.: 25/2016/UDCAW) 40 male Crl:WI rats were randomly divided into control (n=10), bioplast (n=10), PACAP (n=10), and PACAP + bioplast (n=10) groups. Under general anesthesia the right femoral artery was dissected and anastomosed using end-to-end anastomosis and simple interrupted stitches. Blood was drawn from the lateral tail vein before surgery and every seven days for three weeks. In the second and fourth groups bioplast (Surgicel foam) was used. In the third and fourth PACAP was administered (2 µmol/animal) every two days through a subcutaneous canula. The microcirculation of the hind limbs was measured using a Laser Doppler flowmeter. From the blood samples the hematological and hemorheological values were calculated. RESULTS: The use of PACAP resulted an increase in the erythrocyte aggregation (M 5 sec). The used of PACAP showed increase in the WBC count over time. The use of PACAP and the bioplast together resulted in a significant increase in the platelet count. The microcirculation of the hind limbs was significantly higher at the end of the follow up period compared to the other groups. CONCLUSION: In conclusion we can say that PACAP has not only local but systemic effects too, because it caused significant changes in both hematological and hemorheological values as well. These findings were supported by the microcirculatory alterations.