This thesis describes the synthesis of a fluorinated mannobioside as a probe for lectin–carbohydrate interactions. A fluorine atom was introduced at the C-2 position of mannose to study its effect on molecular recognition. The synthesis involved protected intermediates, triflate-mediated fluorination, and thioglycoside-based glycosylation. The target compound is intended for binding studies with mannose-specific lectins such as MBL and DC-SIGN. This work contributes to the use of fluorinated glycans as tools to investigate carbohydrate–protein interactions.