Effects of nutrient supply and rapamycin treatment on gene expressions of mTOR signalling pathway and the development of Japanese quail
| dc.contributor.advisor | Czeglédi , Levente | |
| dc.contributor.advisor | Lendvai, Ádám Zoltán | |
| dc.contributor.author | Ndunguru, Sawadi Fransisco | |
| dc.contributor.department | Állattenyésztési tudományok doktori iskola | hu |
| dc.contributor.submitterdep | Mezőgazdaság-, Élelmiszertudományi és Környezetgazdálkodási Kar | |
| dc.date.accessioned | 2026-02-02T09:52:54Z | |
| dc.date.available | 2026-02-02T09:52:54Z | |
| dc.date.defended | 2026-02-23 | |
| dc.date.issued | 2025 | |
| dc.description.abstract | The study investigates the effects of amino acids methionine and leucine supplementation on a restricted diet, effects of dietary restriction on egg components, effects of in ovo amino acids, methionine and leucine supplementation on the mechanism controlling growth and gene expression in the mTOR signalling pathway and the effect of rapamycin treatment on growth and mTOR gene expression and immune genes in apanese quail (Coturnix japonica). It was observed that amino acid (methionine and leucine) supplements can counteract the negative effects of feed restriction on birds. Specifically, supplementing a bird's diet with leucine or methionine can improve its body weight, reproductive health, and total antioxidant capacity, even during a 20% feed restriction. This suggests that the right nutritional composition, especially essential amino acids, is key to reversing the adverse effects of limited food intake on bird growth and reproduction. The research also found that the duration and timing of feed restriction impact egg quality. While a 20%, 30% or 40% reduction in food intake negatively affected egg components, an unpredictable feed access did not. This unpredictable feeding schedule maintained the quality of the yolk, albumin, and eggshell. It kept the yolk-to-albumin ratio stable, suggesting that this method could be a viable strategy to maintain egg quality and improve overall productivity in the poultry industry. Additionally, the study explored the effects of supplementing a bird's diet with amino acids during the embryonic stage. A 1 mg dose of methionine or a 2.5 mg dose of leucine during this period stimulated the nutrient-sensing pathway, leading to increased postnatal growth. These findings indicate that early nutritional intervention with specific amino acids can improve growth performance, suggesting a link between embryonic nutrition and a bird's later development. Finally, the research looked at the effects of rapamycin, a drug often used to suppress the immune system. The study found that while rapamycin didn't significantly affect body weight, it did reduce spleen weight, which is associated with a more stable immune response. The drug also had sex-specific effects, with male birds showing a decrease in a growth-related gene (IGF1) and an increase in an immune-related gene (MyD88) compared to females. This indicates that rapamycin could be used to modulate the immune system in birds. | |
| dc.format.extent | 154 | |
| dc.identifier.uri | https://hdl.handle.net/2437/403913 | |
| dc.language.iso | en | |
| dc.subject | Amino acid, growth, mTOR, IGF1, rapamycin, methionine, leucine, dietary restriction, egg components, Japanese quail | |
| dc.subject.discipline | Állattenyésztési tudományok | hu |
| dc.subject.sciencefield | Agrártudományok | hu |
| dc.title | Effects of nutrient supply and rapamycin treatment on gene expressions of mTOR signalling pathway and the development of Japanese quail | |
| dc.title.translated | Effects of nutrient supply and rapamycin treatment on gene expressions of mTOR signalling pathway and the development of Japanese quail | |
| dc.type | PhD, doktori értekezés | hu |
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