The dissertation provides an overview of advancements in high-content screening techniques, with special focus on slide-based imaging cytometry. Contemporary studies that utilized this technology have been highlighted. The review has discussed both the significances, benefits as well as limitations of high-content and high-throughput analyses in different investigations, namely cell cycle analysis, immunophenotyping, quantitative histo-cyto-pathology, cell health analysis, and pharmacology/toxicology. In addition to the review sections, the thesis has also demonstrated four studies that were conducted by the author and his collaborations: High-content analysis of adipocyte differentiation; High-throughput screening of protein-protein interactions; Detection of DNA damage in differentiating cells; and Study of polarization signaling pathway in dendritics. These studies exemplified the successful adoptions of laser scanning cytometry into studying cell biology. Moreover, these studies have shown valuable results with both methodological and biological significance. Namely, the author has shown improved cell recognition in heterogeneous, attached cell culture at high confluence; the valuable application of textural analysis in inspection of cellular morphology; pixel-by-pixel FRET map of protein heterodimerization in large number of attached cells; and proofs of the accumulation of low-grade DNA damage during cell differentiation. The demonstrated applications belong to various orientations and are indeed excellent representatives of objective quantitative cytometry. The establishment of such useful screening platform of the workgroup is encouraging and of great potential, which can effectively serve multiple purposes across different research fields in biology and health cares.