Perspectives of Imaging Cytometry and its Implication in High Content Screening

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dc.contributor.advisorBacsó, Zsolt
dc.contributor.authorMinh, Doan
dc.contributor.departmentMolekuláris sejt- és immunbiológia doktori iskolahu
dc.contributor.submitterdepDE--Általános Orvostudományi Kar -- Biofizikai és Sejtbiológiai Intézet
dc.date.accessioned2015-01-27T07:46:30Z
dc.date.available2015-01-27T07:46:30Z
dc.date.created2015hu_HU
dc.date.defended2015-01-09
dc.description.abstractThe dissertation provides an overview of advancements in high-content screening techniques, with special focus on slide-based imaging cytometry. Contemporary studies that utilized this technology have been highlighted. The review has discussed both the significances, benefits as well as limitations of high-content and high-throughput analyses in different investigations, namely cell cycle analysis, immunophenotyping, quantitative histo-cyto-pathology, cell health analysis, and pharmacology/toxicology. In addition to the review sections, the thesis has also demonstrated four studies that were conducted by the author and his collaborations: High-content analysis of adipocyte differentiation; High-throughput screening of protein-protein interactions; Detection of DNA damage in differentiating cells; and Study of polarization signaling pathway in dendritics. These studies exemplified the successful adoptions of laser scanning cytometry into studying cell biology. Moreover, these studies have shown valuable results with both methodological and biological significance. Namely, the author has shown improved cell recognition in heterogeneous, attached cell culture at high confluence; the valuable application of textural analysis in inspection of cellular morphology; pixel-by-pixel FRET map of protein heterodimerization in large number of attached cells; and proofs of the accumulation of low-grade DNA damage during cell differentiation. The demonstrated applications belong to various orientations and are indeed excellent representatives of objective quantitative cytometry. The establishment of such useful screening platform of the workgroup is encouraging and of great potential, which can effectively serve multiple purposes across different research fields in biology and health cares.hu_HU
dc.description.correctorde
dc.format.extent72hu_HU
dc.identifier.urihttp://hdl.handle.net/2437/204650
dc.language.isoenhu_HU
dc.subjectHigh content screeninghu_HU
dc.subjectHigh throughput screening
dc.subjectHigh content analysis
dc.subjectimaging cytometry
dc.subjectlaser scanning cytometry
dc.subjectquantitative cytometry
dc.subjectadipocyte
dc.subjectadipogenesis
dc.subjectDNA damage
dc.subjectDNA break
dc.subjecthalo assay
dc.subjectcomet assay
dc.subjectFRET
dc.subjectprotein interaction
dc.subjectdendritic cell
dc.subject.disciplineElméleti orvostudományokhu
dc.subject.sciencefieldOrvostudományokhu
dc.titlePerspectives of Imaging Cytometry and its Implication in High Content Screeninghu_HU
dc.title.translatedPerspectives of Imaging Cytometry and its Implication in High Content Screeninghu_HU
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Molekuláris Sejt- és Immunbiológia Doktori Iskola