In this study the effect of N,P and K nutrients on the S and protein content of wheat grains was investigated in a long-term fertilization experiment set up in Nagyhörcsök. The calcareous chernozem soil having the following characteristics: pH (KCl): 7.3, CaCO 3 : 4.27%, humus: 3.45%, Al-soluble P 2 O 5 and K 2 O: 60–80 and 180–200, KCl- Mg: 150–180, KCl+EDTA-soluble Mn-, Cu- and Zn-content: 80–150, 2–3 and 1–2 mg kg -1 . The experiment had a split-split-plot design with 40 treatments in 4 replications. Plant samples were collected from 2002 and 2004. 2002 was a drought year while 2004 was very wet. The main conclusions are as follows: – The sulphur and protein content were than the control higher in every NPK treatments. – The sulphur and protein content of the wheat grains were higher in 2004 that had a lot of rain than in 2002 that had drouht.